Human TNFA protein (Active)

• Catalog Number: orb359192
• Category: Proteins
• Description: Recombinant human TNFA active protein
• Tag: N-terminal 6xHis-tagged
• Form/Appearance: Lyophilized powder
• Purity: > 97% as determined by SDS-PAGE and HPLC.
• MW: 18.3 kDa
• UniProt ID: P01375
• Protein Sequence: MHHHHHH+VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL
• Protein Length: Partial
• Source: E.Coli
• Expression System: 77-233aa
• Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.05 ng/ml, corresponding to a specific activity of ＞ 2.0 × 107 IU/mg in the presence of actinomycin D.
• Expression Region: 77-233aa
• Endotoxins: Less than 1.0 EU/µg as determined by LAL method.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
• Buffer/Preservatives: Lyophilized from a 0.2 μm filtered PBS, pH 7.0
• Alternative names: Cachectin, Tumor necrosis factor ligand superfamil
• Note: For research use only
• Application notes: This is His protein
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human TNFA protein (Active)