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Catalog Number | orb428814 |
---|---|
Category | Tools |
Description | Recombinant of human,plant TGFB3 protein |
Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Purity | Greater than 95.0% as determined by SDS-PAGE. |
Solubility (25°C) | It is recommended to reconstitute the lyophilized TGFB3 in sterile 5mM HCl & 50ug/ml BSA at a concentration of 0.05mg/ml, which can then be further diluted to other aqueous solutions. |
Protein Sequence | HHHHHALDTNYCFRNLEENCCVRPLYIDFRQDLGWKWVHEPKGYYANFCSGPCPYLRSADTTHSTVLGLYNTLNPEASASPCCVPQDLEPLTILYYVGRTPKVEQLSNMVVKSCKCS |
Source | Nicotiana benthamiana |
Biological Activity | The biological activity of TGFB3 is measured in culture by its ability to inhibit the mink lung epithelial (Mv1Lu) cells proliferation. ED50 ? 40ng/ml corresponding to a specific activity of 25,000 Units/mg. |
Storage | Stability: Lyophilized TGFB3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TGFB3 Human should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles |
Buffer/Preservatives | Lyophilized from a concentrated (1mg/ml) solution containing 50mM Tris-HCl pH-7.4. |
Alternative names | Transforming Growth Factor-beta3, TGFB3, ARVD, FLJ Read more... |
Note | For research use only |
Application notes | Cytokines And Growth Factors |
Expiration Date | 12 months from date of receipt. |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
Greater than 90% as determined by SDS-PAGE. | |
48.1 kDa | |
E.coli |
Greater than 95% as determined by SDS-PAGE. | |
12.7 kDa | |
Mammalian cell |
Greater than 90% as determined by SDS-PAGE. | |
16.7 kDa | |
E.coli |
> 95% by SDS-PAGE. | |
KMP1652, Recombinant Human TGF-beta 3/TGFB3 Protein is produced by Human Cells expression system. The target protein is expressed with sequence (Ala301-Ser412(Tyr340Phe)) of human TGF-beta 3/TGFB3 (Accession #P10600). |
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