Human SEMA3A protein

SKU: orb707046

Description

Recombinant Human Semaphorin 3A Protein is produced by mammalian expression system and the target gene encoding Lys26-Phe546 is expressed with a 6His-Flag tag at the N-terminus.

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, MS, SDS-PAGE, WB
• Application Notes:
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

• Source: Human cells
• Target: SEMA3A
• Reactivity: Human
• Endotoxins: Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Protein Sequence: GSGSHHHHHH DYKDDDDKKN NVPRLKLSYK EMLESNNVIT FNGLANSSSY HTFLLDEERS RLYVGAKDHI FSFDLVNIKD FQKIVWPVSY TRRDECKWAG KDILKECANF IKVLKAYNQT HLYACGTGAF HPICTYIEIG HHPEDNIFKL ENSHFENGRG KSPYDPKLLT ASLLIDGELY SGTAADFMGR DFAIFRTLGH HHPIRTEQHD SRWLNDPKFI SAHLISESDN PEDDKVYFFF RENAIDGEHS GKATHARIGQ ICKNDFGGHR SLVNKWTTFL KARLICSVPG PNGIDTHFDE LQDVFLMNFK DPKNPVVYGV FTTSSNIFKG SAVCMYSMSD VRRVFLGPYA HRDGPNYQWV PYQGRVPYPR PGTCPSKTFG GFDSTKDLPD DVITFARSHP AMYNPVFPMN NRPIVIKTDV NYQFTQIVVD RVDAEDGQYD VMFIGTDVGT VLKVVSIPKE TWYDLEEVLL EEMTVFREPT AISAMELSTK QQQLYIGSTA GVAQLPLHRC DIYGKACAEC CLARDPYCAW DGSACSRYF

Storage & Handling

• Storage: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of PBS, pH 7.4.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SEMAD; Semaphorin-3A; Semaphorin III; Sema III

Quick Database Links

Gene Symbol

SEMA3A

UniProt

Q14563

NCBI Gene ID

10371