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Human RSPO1 protein

Catalog Number: orb594926

DispatchUsually dispatched within 1-2 weeks
$ 260.00
Catalog Numberorb594926
CategoryProteins
DescriptionRecombinant Human R-spondin-1(RSPO1),partial (Active)
TagC-terminal 6xHis-tagged
Form/AppearanceLyophilized powder
Buffer/PreservativesLyophilized from a 0.2 μm filtered 1xPBS, pH 7.4
PurityGreater than 95% as determined by SDS-PAGE.
Protein SequenceSRGIKGKRQRRISAEGSQACAKGCELCSEVNGCLKCSPKLFILLERNDIRQVGVCLPSCPPGYFDARNPDMNKCIKCKIEHCEACFSHNFCTKCKEGLYLHKGRCYPACPEGSSAANGTMECSSPAQCEMSEWSPWGPCSKKQQLCGFRRGSEERTRRVLHAPVGDHAACSDTKETRRCTVRRVPCPEGQKRRKGGQGRRENANRNLARKESKEAGAGSRRRKGQQQQQQQGTVGPLTSAGPA
Protein LengthFull Length of Mature Protein
UniProt IDQ2MKA7
MW27.8 kDa
Application notesPartial
EndotoxinsLess than 1.0 EU/µg as determined by LAL method.
SourceMammalian cell
Biological OriginHomo sapiens (Human)
Biological ActivityThe ED50 as determined by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells is 1-10ng/ml.
Expression Region31-263aa
StorageThe shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
Alternative namesRSPO1; R-spondin1; RP11-566C13.1; CRISTIN3; FLJ409
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NoteFor research use only
Human RSPO1 protein

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

  • Human RSPO1(21-146) Protein, hFc Tag [orb1290875]

    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.

    The protein has a predicted molecular mass of 39.9 kDa after removal of the signal peptide. The apparent molecular mass of RSPO1(21-146)-hFc is approximately 35-55 kDa due to glycosylation.

    Mammalian

    100 μg, 10 μg, 50 μg
  • Human RSPO1 Protein [orb1477849]

    Greater than 95% as determined by SDS-PAGE.

    30.2 kDa

    Mammalian cell

    1 mg, 20 μg, 100 μg
  • Human RSPO1(21-263) Protein, His Tag [orb1290876]

    The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.

    The protein has a predicted molecular mass of 27.6 kDa after removal of the signal peptide. The apparent molecular mass of RSPO1(21-263)-His is approximately 35-55 kDa due to glycosylation.

    Mammalian

    100 μg, 10 μg, 50 μg
  • Human RSPO1 protein [orb392401]

    ELISA,  MS,  SDS-PAGE,  WB

    Greater than 95% as determined by reducing SDS-PAGE.

    Human cells

    500 μg, 50 μg, 10 μg
  • Recombinant human RSPO1 protein, C-His (HEK293) [orb1516340]

    > 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC

    Due to glycosylation, the protein migrates to 38-42 kDa based on Tris-Bis PAGE result.

    50 μg