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Catalog Number | orb392357 |
---|---|
Category | Proteins |
Description | Recombinant human PTH protein is produced by our E. coli. It is a single non-glycosylated polypeptide chain containing 84 amino acids with a molecular mass of 9 kD. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 10mM HAc-NaAc, 150mM NaCl, 5% Mannitol, pH 4.0. |
Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
Target | PTH |
Entrez | 5741 |
UniProt ID | P01270 |
Protein Sequence | SVSEIQLMHN LGKHLNSMER VEWLRKKLQD VHNFVALGAP LAPRDAGSQR PRKKEDNVLV ESHEKSLGEA DKADVNVLTK AKSQ |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 10mM HAc-NaAc, 150mM NaCl, 5% Mannitol, pH 4.0. |
Alternative names | Parathyroid hormone; PTH; Parathormone; Parathyrin Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
> 97% as determined by SDS-PAGE and HPLC. | |
4.1 kDa | |
E.Coli |
> 97% as determined by SDS-PAGE and HPLC. | |
3.4 kDa | |
E.Coli |
> 97% as determined by SDS-PAGE and HPLC. | |
9.4 kDa | |
E.Coli |
Unconjugated | |
85% | |
19.6 kDa | |
Human PTH1R, His Tag (orb257788) is expressed from human 293 cells (HEK293). It contains AA Asp 27 - Gly 188 (Accession # Q03431-1). |
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