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Catalog Number | orb392373 |
---|---|
Category | Proteins |
Description | Recombinant human PFKFB1 protein is produced by our mammalian expression system and the target gene encoding Ser2-Tyr471 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Liquid in a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | PFKFB1 |
Entrez | 5207 |
UniProt ID | P16118 |
Protein Sequence | SPEMGELTQT RLQKIWIPHS SGSSRLQRRR GSSIPQFTNS PTMVIMVGLP ARGKTYISTK LTRYLNWIGT PTKVFNLGQY RREAVSYKNY EFFLPDNMEA LQIRKQCALA ALKDVHNYLS HEEGHVAVFD ATNTTRERRS LILQFAKEHG YKVFFIESIC NDPGIIAENI RQVKLGSPDY IDCDREKVLE DFLKRIECYE VNYQPLDEEL DSHLSYIKIF DVGTRYMVNR VQDHIQSRTV YYLMNIHVTP RSIYLCRHGE SELNIRGRIG GDSGLSVRGK QYAYALANFI QSQGISSLKV WTSHMKRTIQ TAEALGVPYE QWKALNEIDA GVCEEMTYEE IQEHYPEEFA LRDQDKYRYR YPKGESYEDL VQRLEPVIME LERQENVLVI CHQAVMRCLL AYFLDKSSDE LPYLKCPLHT VLKLTPVAYG CKVESIYLNV EAVNTHREKP ENVDITREPE EALDTVPAHY VDHHHHHH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | Liquid in a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Alternative names | F6PK; PFRX; 6-phosphofructo-2-kinase/fructose-2,6- Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
≥90% as determined by SDS-PAGE | |
This protein contains the human PFKFB1(Ser2-Tyr471) was fused with the C-terminal His Tag and expressed in Mammalian cells. |
98.00% | |
60 KDa, reducing conditions |
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