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Catalog Number | orb1743910 |
---|---|
Category | Antibodies |
Description | Anti-PFKFB1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, FC, IHC, WB |
Reactivity | Human |
Immunogen | E.coli-derived human PFKFB1 recombinant protein (Position: R12-R186). |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.25-0.5 μg/ml, Human Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human Direct ELISA, 0.1-0.5 μg/ml, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 55 kDa |
UniProt ID | P16118 |
Storage | At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing. |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of HepG2 cells using anti-PFKFB1 antibody(Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.
WB analysis of PFKFB1 using anti-PFKFB1 antibody.Lane 1:human hepatocellular carcinoma tumor tissue (HCCT) lysates;2:human hepatocellular carcinoma paracancerous tissue (HCCP) lysates.
IHC analysis of PFKFB1 using anti-PFKFB1 antibody. PFKFB1 was detected in a paraffin-embedded section of human liver cancer tissue.
IHC analysis of PFKFB1 using anti-PFKFB1 antibody. PFKFB1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue.
IHC analysis of PFKFB1 using anti-PFKFB1 antibody. PFKFB1 was detected in a paraffin-embedded section of human testicular seminoma tissue.
IHC analysis of PFKFB1 using anti-PFKFB1 antibody. PFKFB1 was detected in a paraffin-embedded section of human tonsil tissue.
ELISA, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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