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Catalog Number | orb594736 |
---|---|
Category | Proteins |
Description | Recombinant Human Platelet-derived growth factor subunit B(PDGFB) (Active) |
Tag | Tag-Free |
Form/Appearance | Lyophilized powder |
Purity | Greater than 98% as determined by SDS-PAGE. |
MW | 12.42 kDa |
UniProt ID | P01127 |
Protein Sequence | SLGSLTIAEPAMIAECKTRTEVFEISRRLIDRTNANFLVWPPCVEVQRCSGCCNNRNVQCRPTQVQLRPVQVRKIEIVRKKPIFKKATVTLEDHLACKCETVAAARPVT |
Protein Length | Full Length of Mature Protein |
Source | E.coli |
Expression System | Expression Region: 82-190aa. Protein Length: Full Length of Mature Protein |
Biological Activity | The ED50 as determined in a cell proliferation assay using BALB/c 3T3 cells is 5-20 ng/ml. |
Expression Region | 82-190aa |
Endotoxins | Less than 0.2 EU/µg as determined by LAL method. |
Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution of 20mM NaAc-HAc, pH 4.5. |
Alternative names | Platelet-Derived Growth Factor Subunit B; PDGF Sub Read more... |
Note | For research use only |
Application notes | Full Length of Mature Protein |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
95% | |
15.1 kDa | |
Unconjugated Human PDGF-BB, His, (orb334919) is expressed from E. coli cells. It contains AA Ser 82 - Thr 190 (Accession # P01127-1). |
Unconjugated | |
90% | |
20.3 kDa | |
Human VEGF-B, His Tag (orb1152444) is expressed from human 293 cells (HEK293). It contains AA Pro 22 - Ala 207 (Accession # P49765-1). |
Greater than 85% as determined by SDS-PAGE. | |
28.3 kDa | |
E.coli |
Greater than 90% as determined by SDS-PAGE. | |
14.3 kDa | |
E.coli |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
E. coli |
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