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Catalog Number | orb640279 |
---|---|
Category | Proteins |
Description | Recombinant human Novel Coronavirus Spike glycoprotein(S), partial protein |
Tag | C-terminal 6xHis-mFc-tagged |
Form/Appearance | Lyophilized powder |
Purity | Greater than 90% as determined by SDS-PAGE. |
MW | 51.1 kDa |
UniProt ID | P0DTC2 |
Protein Sequence | RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNF |
Protein Length | Partial (S1-RBD) |
Source | Mammalian cell |
Expression System | Expression Region: 319-541aa. Protein Length: Partial |
Biological Activity | ①Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 μg/ml can bind Biotinylated Anti-SARS-CoV-2-S Antibody (CSB-RA33245D1GMY), the EC50 of SARS-CoV-2-S1-RBD protein is 106.2-131.2 ng/ml. ②Measured by its binding ability in a functional ELISA. Immobilized human ACE2 (CSB-MP866317HU) at 2 μg/ml can bind SARS-CoV-2-S1-RBD, the EC50 of SARS-CoV-2-S1-RBD protein is 8.363-12.82 ng/ml. |
Expression Region | 319-541aa |
Endotoxins | Less than 1.0 EU/ug as determined by LAL method. |
Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0 |
Alternative names | / Read more... |
Note | For research use only |
Application notes | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
Expiration Date | 6 months from date of receipt. |
Greater than 85% as determined by SDS-PAGE. | |
79.6 kDa | |
Mammalian cell |
Greater than 90% as determined by SDS-PAGE. | |
38.2 kDa | |
Yeast |
Greater than 90% as determined by SDS-PAGE. | |
88.1 kDa | |
Yeast |
Greater than 85% as determined by SDS-PAGE. | |
77.8 kDa | |
Mammalian cell |
Greater than 90% as determined by SDS-PAGE. | |
30.1 kDa | |
Mammalian cell |
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