Human Novel Coronavirus Spike glycoprotein(S) protein (Biotin)

SKU: orb668865

Description

This Human Novel Coronavirus Spike glycoprotein(S) protein (Biotin) spans the amino acid sequence from region 319-541aa. Purity: Greater than 90% as determined by SDS-PAGE.

Research Area

Epigenetics, Infectious Diseases, Non-Animal

Images & Validation

• Application Notes:
  SARS-CoV-2 Related Proteins

Key Properties

• Source: Mammalian cell
• Biological Origin: Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)
• Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized human ACE2 at 2 μg/ml can bind Biotinylated-SARS-CoV-2-S1-RBD, the EC50 is 5.087-7.050 ng/ml.
• Tag: C-terminal mFc-Avi-tagged
• Expression Region: 319-541aa
• Protein Length: Partial
• Endotoxins: Less than 1.0 EU/μg as determined by LAL method.
• MW: 54.1 kDa
• Purity: Greater than 90% as determined by SDS-PAGE.
• Protein Sequence: RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNF

Storage & Handling

• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
• Form/Appearance: Lyophilized powder
• Buffer/Preservatives: Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Measured by its binding ability in a functional ELISA. Immobilized human ACE2 at 2 μg/ml can bind Biotinylated-SARS-CoV-2-S1-RBD, the EC50 is 5.087-7.050 ng/ml

The purity of S was greater than 95% as determined by SEC-HPLC

Quick Database Links

UniProt

P0DTC2