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Human MAP1LC3B protein

SKU: orb706998

Description

Recombinant Human LC3B Protein is produced by E.coli expression system and the target gene encoding Met1-Val125 is expressed.

Images & Validation

Tested ApplicationsELISA, MS, SDS-PAGE, WB
Application Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

SourceE. coli
ReactivityHuman
Protein SequenceGSMPSEKTFK QRRTFEQRVE DVRLIREQHP TKIPVIIERY KGEKQLPVLD KTKFLVPDHV NMSELIKIIR RRLQLNANQA FFLLVNGHSM VSVSTPISEV YESEKDEDGF LYMVYASQET FGMKLSV
PurityGreater than 95% as determined by reducing SDS-PAGE.
EndotoxinsLess than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.

Storage & Handling

StorageLyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Form/AppearanceLyophilized from a 0.2 μm filtered solution of 20mM Tris, 150mM NaCl, 2mM DTT, pH 8.0.
DisclaimerFor research use only

Alternative Names

MAP1ALC3; Microtubule-associated proteins 1A/1B light chain 3B; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2; MAP1A/MAP1B light chain 3 B; MAP1A/MAP1B LC3 B; Microtubule-associated protein 1 light chain 3 beta

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Human MAP1LC3B protein (orb706998)

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Available Sizes

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10 μg
$ 250.00
50 μg
$ 600.00
500 μg
$ 2,760.00
DispatchUsually dispatched within 5-10 working days
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