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Catalog Number | orb605229 |
---|---|
Category | Proteins |
Description | Recombinant Human NKG2-D type II integral membrane protein(KLRK1),partial |
Tag | C-terminal hFc-tagged |
Form/Appearance | Lyophilized powder |
Purity | Greater than 93% as determined by SDS-PAGE. |
MW | 43.6 kDa |
UniProt ID | P26718 |
Protein Sequence | FLNSLFNQEVQIPLTESYCGPCPKNWICYKNNCYQFFDESKNWYESQASCMSQNASLLKVYSKEDQDLLKLVKSYHWMGLVHIPTNGSWQWEDGSILSPNLLTIIEMQKGDCALYASSFKGYIENCSTPNTYICMQRTV |
Protein Length | Partial |
Source | Mammalian cell |
Expression System | Expression Region: 78-216aa. Protein Length: Partial |
Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized KLRK1 at 10 μg/ml can bind human ULBP1 (CSB-MP887177HU), the EC50 of human KLRK1 protein is 222.4-276.0 ng/ml. |
Expression Region | 78-216aa |
Endotoxins | Less than 1.0 EU/ug as determined by LAL method. |
Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4 |
Alternative names | Killer cell lectin-like receptor subfamily K membe Read more... |
Note | For research use only |
Application notes | Partial |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
95% | |
33.8 kDa | |
Human MICA, His Tag (orb257689) is expressed from human 293 cells (HEK293). It contains AA Glu 24 - Gln 308 (Accession # AAH16929.1). |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
Unconjugated | |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 42.8 kDa after removal of the signal peptide.The apparent molecular mass of mFc-NKG2D is approximately 50-65 kDa due to glycosylation. | |
Mammalian |
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