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Human IL 1 alpha Protein

Human IL 1 alpha Protein

Catalog Number: orb429395

DispatchUsually dispatched within 5-10 working days
$ 250.00
Catalog Numberorb429395
CategoryProteins
DescriptionRecombinant of human IL1 alpha protein
Form/AppearanceSterile Filtered White lyophilized (freeze-dried) powder.
PurityGreater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Solubility (25°C)It is recommended to reconstitute the lyophilized Interleukin 1 alpha in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Protein SequenceSAPFSFLSNVKYNFMRIIKYEFILNDALNQSIIRANDQYLTAAALHNLDEAV KFDMGAYKSSKDDAKITVILRISKTQLYVTAQDEDQPVLLKEMPEIPKTITG SETNLLFFWETHGTKNYFTSVAHPNLFIATKQDYWVCLAGGPPSITDFQILE NQA
SourceEscherichia Coli
Biological ActivityThe ED50 as determined by the dose-dependant stimulation of murine D10S cells is < 0.001 ng/ml, corresponding to a Specific Activity of 1 x 109 IU/mg.
StorageStability: Lyophilized Interleukin-1 alpha although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL-1a should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles
Buffer/PreservativesThe protein was lyophilized from a concentrated (1mg/ml) sterile solution containing 25mM Tris-HCl, pH 8.
Alternative namesHematopoietin-1, Lymphocyte-activating factor (LAF
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NoteFor research use only
Application notesProtein content: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 1.13 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-1 as a Reference Standard
Expiration Date6 months from date of receipt.
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    Unconjugated

    90%

    39.0 kDa

    Human IL-13 R alpha 2, His Tag (orb612134) is expressed from human 293 cells (HEK293). It contains AA Asp 27 - Arg 343 (Accession # Q14627-1).

    100 μg, 1 mg
  • Human IL-6 R alpha Protein, His Tag [orb257625]

    Unconjugated

    95%

    39.4 kDa

    Human IL-6 R alpha, His Tag (orb257625) is expressed from human 293 cells (HEK293). It contains AA Leu 20 - Pro 365 (Accession # NP_000556.1).

    1 mg, 50 μg
  • Human IL12A protein [orb419321]

    > 95% as determined by SDS-PAGE and HPLC.

    57.2 kDa. 75.0 kDa is the observed value of non-reducing gel,and the reducing glue is the size of p40 and p35 subunits, and 57.2 kDa is the theoretical value.

    Baculovirus

    10 μg, 100 μg, 500 μg
  • Human IL1 alpha protein (Active) [orb358965]

    > 97% as determined by SDS-PAGE and HPLC.

    18.0 kDa

    E.Coli

    500 μg, 10 μg, 100 μg
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    Unconjugated

    90%

    37.7 kDa

    Human IL-13 R alpha 1 Protein, His Tag (orb257580) is expressed from human 293 cells (HEK293). It contains AA Gly 22 - Thr 343 (Accession # NP_001551.1).

    1 mg, 100 μg