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Item 1 of 6
Human IL-13 R alpha 2 Protein, His Tag
Catalog Number: orb612134
| Catalog Number | orb612134 |
|---|---|
| Category | Proteins |
| Description | Human IL-13 R alpha 2, His Tag is expressed from human 293 cells (HEK293). It contains AA Asp 27 - Arg 343 (Accession # Q14627-1). |
| Target | IL-13 R alpha 2 |
| Conjugation | Unconjugated |
| Tag | C-10×His |
| Reactivity | Human |
| Form/Appearance | Powder |
| Buffer/Preservatives | PBS, pH 7.4 |
| Purity | 90% |
| Protein Sequence | NP_000631.1 |
| UniProt ID | Q14627 |
| MW | 39.0 kDa |
| Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 39.0 kDa. The protein migrates as 45-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expression System | HEK293 |
| Endotoxins | 1.0 EU per μg |
| Biological Origin | Human |
| Biological Activity | Loaded Human IL-13, Fc Tag (Cat. No. IL3-H5256) on Protein A Biosensor, can bind Human IL-13 R alpha 2, His Tag (Cat. No. ) with an affinity constant of 6.09 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). |
| Expression Region | Asp 27 - Arg 343 |
| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
| Note | For research use only |
| NCBI | NP_000631.1 |
Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of Human IL-13 R alpha 2 Protein.
Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of Human IL-13 R alpha 2 Protein.
SDS-PAGE analysis of Human IL-13 R alpha 2 Protein.
Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of Human IL-13 R alpha 2 Protein.
Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of Human IL-13 R alpha 2 Protein.
Loaded Human IL-13, Fc Tag (Cat. No. orb612113) on Protein A Biosensor, can bind Human IL-13 R alpha 2, His Tag (Cat. No. orb612134) with an affinity constant of 6.09 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
IL-13RA1 Protein, Human, Recombinant (His & Avi), Biotinylated [orb1960613]
98.00%
39.5 kDa (predicted). Due to glycosylation, the protein migrates to 55-75 kDa based on Tris-Bis PAGE result.
1 mg, 500 μg, 100 μgRecombinant Human IL-13 R alpha 2 Protein, hFc His Tag [orb1550474]
> 95% by Tris-Bis PAGE, > 95% by SEC-HPLC
Recombinant Human IL-13 R alpha 2 Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Asp27-Leu342 ) of Human IL-13 R alpha 2 fused with hFc His Tag tag at the C-terminal.
100 μg
