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Human IHH protein (Active)

Catalog Number: orb359262

DispatchUsually dispatched within 1-2 weeks
$ 210.00
Catalog Numberorb359262
CategoryProteins
DescriptionRecombinant human IHH active protein
TagTag-Free
Form/AppearanceLyophilized powder
Buffer/PreservativesLyophilized from a 0.2 μm filtered 1 × PBS, pH 7.4
Purity> 96% as determined by SDS-PAGE and HPLC.
Protein SequenceII+GPGRVVGSRRRPPRKLVPLAYKQFSPNVPEKTLGASGRYEGKIARSSERFKELTPNYNPDIIFKDEENTGADRLMTQRCKDRLNSLAISVMNQWPGVKLRVTEGWDEDGHHSEESLHYEGRAVDITTSDRDRNKYGLLARLAVEAGFDWVYYESKAHVHCSVKSEHSAAAKTGG
Protein LengthPartial
UniProt IDQ14623
MW19.8 kDa
Application notesWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
EndotoxinsLess than 1.0 EU/µg as determined by LAL method.
SourceE.Coli
Biological OriginHomo sapiens (Human)
Biological ActivityFully biologically active when compared to standard. The ED50 as determined by its ability to induce alkaline phosphatase production by C3H10T1/2(CCL-226) cells is 3.0-10 μg/ml.
Expression Region29-202aa
StorageThe shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
Alternative namesIHH,
NoteFor research use only
Human IHH protein (Active)

SDS-PAGE analysis of Human IHH protein (Active)

  • Recombinant Human Indian hedgehog protein(IHH) (Active) [orb1650679]

    5 μg, 25 μg, 100 μg, 1 mg, 250 μg, 500 μg
  • Recombinant Human Sonic HedgeHog (Shh) [orb1494940]

    > 98% by SDS-PAGE and HPLC analyses.

    Approximately 20.0 kDa, a single non-glycosylated polypeptide chain containing 176 amino acids.

    Escherichia coli

    1 mg, 25 μg, 5 μg
  • RecombinantIHH,Human [orb1494835]

    > 95% as analyzed by SDS-PAGE.

    20 kDa, observed by reducing SDS-PAGE.

    Escherichia coli.

    50 μg, 10 μg, 1 mg
  • RecombinantShh,Mouse(CHO-expressed) [orb1494667]

    > 95% as analyzed by SDS-PAGE and HPLC.

    20 kDa, observed by non-reducing SDS-PAGE.

    CHO

    50 μg, 10 μg, 1 mg