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Human IgM (mu chain) Antibody Alkaline Phosphatase Conjugated
Catalog Number: orb347236
| Catalog Number | orb347236 |
|---|---|
| Category | Antibodies |
| Description | Human IgM (mu chain) antibody (Alkaline Phosphatase) |
| Clonality | Polyclonal |
| Species/Host | Goat |
| Isotype | IgG |
| Conjugation | AP |
| Reactivity | Human |
| Form/Appearance | Liquid (sterile filtered) |
| Concentration | 1.0 mg/mL |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Purity | Anti-Human IgM mu heavy chain antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Human IgM and Human Serum. No reaction was observed against other human heavy or light chain proteins. |
| Immunogen | Human IgM whole molecule |
| Tested applications | DOT, ELISA, IHC, WB |
| Dilution range | ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500 |
| Application notes | Anti-Human IgM (mu heavy chain) Alkaline Phosphatase has been tested by dot blot and ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody based enzymatic assays requiring lot-to-lot consistency. |
| Antibody Type | Secondary Antibody |
| Storage | Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. |
| Alternative names | goat anti-Human IgM mu heavy chain Antibody, Gt-a- Read more... |
| Note | For research use only |
Dot Blot of Goat anti-Human IgM (mu chain) Antibody Alkaline Phosphatase Conjugated. Antigen: Human IgM. Load: Lane 1 - 200 ng Lane 2 - 66.67 ng Lane 3 - 22.22 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: none. Secondary antibody: Goat anti-Human IgM (mu chain) Antibody Alkaline Phosphatase Conjugated at 1:1000 for 60 min at RT. Block: orb348637 for 1 HR at RT. Visualized using Alkaline Phosphatase Substrate for 30 seconds at RT.
