Human IgM (mu chain) Antibody Peroxidase Conjugated

• Catalog Number: orb347226
• Category: Antibodies
• Description: Human IgM (mu chain) antibody (Peroxidase)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Human IgM whole molecule
• Antibody Type: Secondary Antibody
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:180,000, IHC: 1:500 - 1:2,500, WB: 1:2,000 - 1:20,000
• Form/Appearance: Lyophilized
• Purity: Human IgM (mu chain) Antibody, Peroxidase Conjugated, was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Human IgM and Human Serum. No reaction was observed against other human heavy or light chain proteins.
• Conjugation: HRP
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Alternative names: Goat Anti-Human IgM (mu chain) Antibody Peroxidase
• Note: For research use only
• Application notes: Anti-Human IgM (mu heavy chain) Peroxidase has been tested by ELISA and is suitable for western blot, immunohistochemistry, and immunoassays where specificity to the immunoglobulin mu chain region is desired. Optimal concentrations in immunoassays should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

ELISA Results of purified Goat Anti-Human IgM Peroxidase Conjugated Antibody tested against purified Human IgM. The working dilution for Anti-Human IgM HRP is 1:120000. Each well was coated in duplicate with 1.0 µg of Human IgM (p/n orb2652752). The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer and orb348651 substrate.