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Description
Research Area
Images & Validation
−| Tested Applications | ELISA, SDS-PAGE, WB |
|---|
Key Properties
−| Source | Human serum IgG digested with papain |
|---|---|
| Biological Origin | Human serum IgG digested with papain |
| Target | Human IgG Fc fragment |
| Protein Length | Full length protein |
| Purity | > 90%(SDS-PAGE) |
Storage & Handling
−| Storage | Aliquot and store at -20℃ or -80℃. Avoid repeated freeze/thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Hazard Information | For research use only |
| Disclaimer | For research use only |
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HUMAN IgG F(c) was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Serum, anti-Human IgG and anti-Human IgG F(c). No reaction was observed against anti-Human IgG F(ab’)2 or anti-Papain.
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Human IgG Fc Fragment protein (orb527413)
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