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Catalog Number | orb391858 |
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Category | Proteins |
Description | Recombinant human HPD protein is produced by our E. coli expression system and the target gene encoding Met1-Met393 is expressed with a 6His tag at the N-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 50mM NaCl, 1mM DTT, 20% Glycerol, pH 8.0. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | HPD |
Entrez | 3242 |
UniProt ID | P32754 |
Protein Sequence | MGSSHHHHHH SSGLVPRGSH MTTYSDKGAK PERGRFLHFH SVTFWVGNAK QAASFYCSKM GFEPLAYRGL ETGSREVVSH VIKQGKIVFV LSSALNPWNK EMGDHLVKHG DGVKDIAFEV EDCDYIVQKA RERGAKIMRE PWVEQDKFGK VKFAVLQTYG DTTHTLVEKM NYIGQFLPGY EAPAFMDPLL PKLPKCSLEM IDHIVGNQPD QEMVSASEWY LKNLQFHRFW SVDDTQVHTE YSSLRSIVVA NYEESIKMPI NEPAPGKKKS QIQEYVDYNG GAGVQHIALK TEDIITAIRH LRERGLEFLS VPSTYYKQLR EKLKTAKIKV KENIDALEEL KILVDYDEKG YLLQIFTKPV QDRPTLFLEV IQRHNHQGFG AGNFNSLFKA FEEEQNLRGN LTNMETNGVV PGM |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 50mM NaCl, 1mM DTT, 20% Glycerol, pH 8.0. |
Alternative names | PPD; 4-hydroxyphenylpyruvate dioxygenase; 4-hydrox Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
Greater than 95% as determined by SDS-PAGE. | |
52.7 kDa | |
Mammalian cell |
Greater than 93% as determined by SDS-PAGE. | |
18.2 kDa | |
Mammalian cell |
Greater than 90% as determined by SDS-PAGE. | |
48.8 kDa | |
E.coli |
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