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Human GCGR protein
Description
Images & Validation
−| Application Notes |
|---|
Key Properties
−| Source | Mammalian cell |
|---|---|
| Biological Origin | Homo sapiens (Human) |
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized human GCGR at 2 μg/mL can bind Anti-GCGR recombinant antibody, the EC50 is 3.747-6.666 ng/mL. |
| Tag | N-terminal 10xHis-tagged and C-terminal Myc-tagged |
| Molecular Weight | 18.1 kDa |
| Expression Region | 26-136aa |
| Protein Length | Partial |
| Protein Sequence | AQVMDFLFEKWKLYGDQCHHNLSLLPPPTELVCNRTFDKYSCWPDTPANTTANISCPWYLPWHHKVQHRFVFKRCGPDGQWVRGPRGQPWRDASQCQMDGEEIEVQKEVAK |
| Purity | Greater than 95% as determined by SDS-PAGE. |
| Endotoxins | Less than 1.0 EU/ug as determined by LAL method. |
Storage & Handling
−| Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
|---|---|
| Form/Appearance | Lyophilized powder |
| Buffer/Preservatives | Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0 |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Human GCGR Protein, hFc Tag [orb1290961]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 39.2 kDa after removal of the signal peptide. The apparent molecular mass of GCGR-hFc is approximately 55-70 kDa due to glycosylation.
Mammalian
100 μg, 50 μg, 10 μgHuman GCGR full length protein-synthetic nanodisc [orb1743186]
The human full length GCGR protein has a MW of 54.0 kDa
Mammalian
10 μg, 50 μg, 100 μgHuman GCGR protein [orb594980]
Greater than 85% as determined by SDS-PAGE.
16.9 kDa
Baculovirus
1 mg, 20 μg, 100 μgHuman GCGR protein [orb605329]
Greater than 90% as determined by SDS-PAGE.
15.1 kDa
Yeast
1 mg, 100 μg, 20 μg

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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Measured by its binding ability in a functional ELISA. Immobilized human GCGR at 2 μg/ml can bind Anti-GCGR recombinant antibody, the EC50 is 3.747-6.666 ng/mL.
Quick Database Links
UniProt Details
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Protocol Information
Human GCGR protein (orb605217)
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