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Catalog Number | orb391610 |
---|---|
Category | Proteins |
Description | Recombinant human DCK protein is produced by our E. coli expression system and the target gene encoding Met1-Leu260 is expressed with a 6His, T7 tag at the N-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, pH 7.5. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | DCK |
Entrez | 1633 |
UniProt ID | P27707 |
Protein Sequence | MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSMATPPK RSCPSFSASS EGTRIKKISI EGNIAAGKST FVNILKQLCE DWEVVPEPVA RWCNVQSTQD EFEELTMSQK NGGNVLQMMY EKPERWSFTF QTYACLSRIR AQLASLNGKL KDAEKPVLFF ERSVYSDRYI FASNLYESEC MNETEWTIYQ DWHDWMNNQF GQSLELDGII YLQATPETCL HRIYLRGRNE EQGIPLEYLE KLHYKHESWL LHRTLKTNFD YLQEVPILTL DVNEDFKDKY ESLVEKVKEF LSTL |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, pH 7.5. |
Alternative names | Deoxycytidine kinase; dCK Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Greater than 90% as determined by SDS-PAGE. | |
31.6 kDa | |
Yeast |
Greater than 85% as determined by SDS-PAGE. | |
35.5 kDa | |
E.coli |
≥90% as determined by SDS-PAGE | |
This protein contains the human DCK(Met1-Leu260) was fused with the N-terminal His, T7 Tag and expressed in E. coli. |
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