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Catalog Number | orb750336 |
---|---|
Category | Proteins |
Description | Human Tissue Factor, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Ser 33 - Glu 251 (Accession # P13726-1). |
Target | Coagulation Factor III |
Conjugation | Unconjugated |
Tag | C-Fc |
Reactivity | Human |
Form/Appearance | Powder |
Buffer/Preservatives | 50 mM Tris, 150 mM NaCl, pH 7.5 |
Purity | 95% |
Protein Sequence | NP_001984.1 |
UniProt ID | P13726 |
MW | 51.2 kDa |
Application notes | This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 51.2 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expression System | HEK293 |
Endotoxins | 1.0 EU per μg |
Biological Origin | Human |
Expression Region | Ser 33 - Glu 251 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
NCBI | NP_001984.1 |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 50.9 kDa after removal of the signal peptide. The apparent molecular mass of CD142-hFc is approximately 55-70kDa due to glycosylation. | |
Mammalian |
> 92%, determined by SDS-PAGE | |
This protein contains the human F3 (P13726) extracellular domain (Met 1-Glu 251) was fused with the Fc region of human IgG1 at the C-terminus and expressed from HEK293 Cells. |