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| Catalog Number | orb753018 |
|---|---|
| Category | Proteins |
| Description | CA8 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 314 amino acids (1-290) and having a molecular mass of 35.5kDa. CA8 Humanis fused to a 24 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques. |
| Form/Appearance | Sterile Filtered clear solution. |
| Buffer/Preservatives | CA8 Human protein (1mg/ml) is formulated in 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 1mM DTT. |
| Purity | Greater than 90.0% as determined by SDS-PAGE. |
| Protein Sequence | MGSSHHHHHH SSGLVPRGSH MGSHMADLSF IEDTVAFPEK EEDEEEEEEG VEWGYEEGVEWGLVFPDANG EYQSPINLNS REARYDPSLL DVRLSPNYVV CRDCEVTNDG HTIQVILKSKSVLSGGPLPQ GHEFELYEVR FHWGRENQRG SEHTVNFKAF PMELHLIHWN STLFGSIDEAVGKPHGIAII ALFVQIGKEH VGLKAVTEIL QDIQYKGKSK TIPCFNPNTL LPDPLLRDYWVYEGSLTIPP CSEGVTWILF RYPLTISQLQ IEEFRRLRTH VKGAELVEGC DGILGDNFRP TQPLSDRVIR AAFQ |
| Application notes | Enzymes |
| Source | Escherichia Coli |
| Biological Activity | Specific activity is > 450 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C. |
| Storage | Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles |
| Alternative names | Carbonic anhydrase-related protein, CA-VIII, CALS, Read more... |
| Note | For research use only |
| Expiration Date | 6 months from date of receipt. |
Unconjugated | |
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. | |
Predicted: 34.04 KDa. Observed: 40 KDa, reducing conditions |
Greater than 90% as determined by SDS-PAGE. | |
60 kDa | |
E.coli |
Greater than 95% as determined by SDS-PAGE. | |
34.04 kDa | |
E.coli |
>90% as determined by SDS-PAGE. | |
35.28 kDa |
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