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Catalog Number | orb1292402 |
---|---|
Category | Proteins |
Description | Recombinant Human CA12 protein |
Tested applications | ELISA, WB |
Reactivity | Human |
Tag | N-terminal His-IF2DI Tag |
Purity | Greater than 95% by SDS-PAGE gel analyses |
MW | 49.9 kDa |
Formula | Lyophilized from a 0.2 μm filtered solution in PBS with 5% trehalose, pH7.4 |
UniProt ID | O43570 |
Source | E.Coli |
Expression System | 25-283 |
Storage | -20°C for 12 months as lyophilized;2-8°C for 1 month under sterile conditions after reconstitution |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution in PBS with 5% trehalose, pH7.4 |
Alternative names | CA XII, CA12, Carbonate dehydratase XII, Carbonic Read more... |
Note | For research use only |
Application notes | Centrifuge the vial at 10,000 rpm for 1 minute, reconstitute at 200 μg/ml in sterile distilled water by gentle pipetting 2-3 times, don’t vortex |
Expiration Date | 6 months from date of receipt. |
Greater than 90% as determined by SDS-PAGE. | |
33.1 kDa | |
Yeast |
Greater than 90% as determined by SDS-PAGE. | |
47.1 kDa | |
E.coli |
Unconjugated | |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 57.3 kDa after removal of the signal peptide. The apparent molecular mass of CA12-hFc is approximately 55-70 kDa due to glycosylation. | |
Mammalian |
> 90% by SDS-PAGE. | |
KMP1153, Recombinant Human Carbonic Anhydrase XII/CA12 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Ala25-Gln291) of human Carbonic Anhydrase XII/CA12 (Accession #NP_001209.1) fused with a 6×His Tag at the C-terminus. |
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