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Catalog Number | orb245772 |
---|---|
Category | Proteins |
Description | Recombinant human Carbonic anhydrase 12 |
Tag | N-terminal 6xHis-tagged |
Form/Appearance | Liquid or Lyophilized powder |
Purity | Greater than 90% as determined by SDS-PAGE. |
MW | 33.1 kDa |
UniProt ID | O43570 |
Protein Sequence | APVNGSKWTYFGPDGENSWSKKYPSCGGLLQSPIDLHSDILQYDASLTPLEFQGYNLSANKQFLLTNNGHSVKLNLPSDMHIQGLQSRYSATQLHLHWGNPNDPHGSEHTVSGQHFAAELHIVHYNSDLYPDASTASNKSEGLAVLAVLIEMGSFNPSYDKIFSHLQHVKYKGQEAFVPGFNIEELLPERTAEYYRYRGSLTTPPCNPTVLWTVFRNPVQISQEQLLALETALYCTHMDDPSPREMINNFRQVQKFDERLVYTSFSQVQVCTAAGLS |
Protein Length | Extracellular Domain |
Source | Yeast |
Expression System | Expression Region: 25-301aa. Protein Length: Extracellular Domain |
Expression Region | 25-301aa |
Endotoxins | Not test. |
Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
Buffer/Preservatives | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Alternative names | CA12 Read more... |
Note | For research use only |
Application notes | This is His-tag protein |
Expiration Date | 6 months from date of receipt. |
Greater than 90% as determined by SDS-PAGE. | |
47.1 kDa | |
E.coli |
Unconjugated | |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 57.3 kDa after removal of the signal peptide. The apparent molecular mass of CA12-hFc is approximately 55-70 kDa due to glycosylation. | |
Mammalian |
> 90% by SDS-PAGE. | |
KMP1153, Recombinant Human Carbonic Anhydrase XII/CA12 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Ala25-Gln291) of human Carbonic Anhydrase XII/CA12 (Accession #NP_001209.1) fused with a 6×His Tag at the C-terminus. |
ELISA, WB | |
Greater than 95% by SDS-PAGE gel analyses | |
49.9 kDa | |
E.Coli |
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