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Catalog Number | orb706847 |
---|---|
Category | Proteins |
Description | Recombinant Human CD166 Protein is produced by mammalian expression system and the target gene encoding Trp28-Ala526 is expressed with a Fc tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | ALCAM |
Entrez | 214 |
UniProt ID | Q13740 |
Protein Sequence | WYTVNSAYGD TIIIPCRLDV PQNLMFGKWK YEKPDGSPVF IAFRSSTKKS VQYDDVPEYK DRLNLSENYT LSISNARISD EKRFVCMLVT EDNVFEAPTI VKVFKQPSKP EIVSKALFLE TEQLKKLGDC ISEDSYPDGN ITWYRNGKVL HPLEGAVVII FKKEMDPVTQ LYTMTSTLEY KTTKADIQMP FTCSVTYYGP SGQKTIHSEQ AVFDIYYPTE QVTIQVLPPK NAIKEGDNIT LKCLGNGNPP PEEFLFYLPG QPEGIRSSNT YTLTDVRRNA TGDYKCSLID KKSMIASTAI TVHYLDLSLN PSGEVTRQIG DALPVSCTIS ASRNATVVWM KDNIRLRSSP SFSSLHYQDA GNYVCETALQ EVEGLKKRES LTLIVEGKPQ IKMTKKTDPS GLSKTIICHV EGFPKPAIQW TITGSGSVIN QTEESPYING RYYSKIIISP EENVTLTCTA ENQLERTVNS LNVSAISIPE HDEADEISDE NREKVNDQAD IEGRMDPKSC DKTHTCPPCP APELLGGPSV FLFPPKPKDT LMISRTPEVT CVVVDVSHED PEVKFNWYVD GVEVHNAKTK PREEQYNSTY RVVSVLTVLH QDWLNGKEYK CKVSNKALPA PIEKTISKAK GQPREPQVYT LPPSRDELTK NQVSLTCLVK GFYPSDIAVE WESNGQPENN YKTTPPVLDS DGSFFLYSKL TVDKSRWQQG NVFSCSVMHE ALHNHYTQKS LSLSPGK |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
Alternative names | MEMD protein, CD166 antigen protein, Activated leu Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
95% | |
57.8 kDa | |
Human ALCAM, His Tag (orb257286) is expressed from human 293 cells (HEK293). It contains AA Trp 28 - Ala 526 (Accession # Q13740-1). |
Unconjugated | |
95% | |
82.6 kDa | |
Human ALCAM, Fc Tag (orb257290) is expressed from human 293 cells (HEK293). It contains AA Trp 28 - Ala 526 (Accession # NP_001618). |
Human | |
0.039 ng/mL-2.5 ng/mL | |
0.0097 ng/mL |
Greater than 90% as determined by SDS-PAGE. | |
70.8 kDa | |
E.coli |
≥90% as determined by SDS-PAGE | |
This protein contains the human ALCAM(Trp28-Ala526) was fused with the C-terminal Fc Tag and expressed in Mammalian cells. |
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