HSPA8 Antibody (N-term)

• Catalog Number: orb1931377
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB17655
• Tested applications: FC, IF, IHC-P, WB
• Predicted Reactivity: Equine, Hamster
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Dilution range: IF: 1:10~50, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 70898 Da
• Target: This HSPA8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 82-110 amino acids from the N-terminal region of human HSPA8.
• UniProt ID: P11142
• NCBI: NP_006588.1, NP_694881.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Heat shock cognate 71 kDa protein, Heat shock 70 k
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

HSPA8 Antibody (N-term) flow cytometric analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of HSPA8 Antibody (N-term) with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Formalin-fixed and paraffin-embedded human brain tissue reacted with HSPA8 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of lysates from A431, Hela, mouse NIH/3T3, rat H-4-II-E cell line (from left to right), using HSPA8 Antibody (N-term).Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.