HSP70-2 Antibody / HSPA1B

SKU: orb1823256

Description

This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1.The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq]

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the HSP70-2 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b, kappa
• Clone No.: HSPA1B/7625
• Immunogen: A recombinant partial protein sequence (within amino acids 400-600) from the human protein was used as the immunogen for the HSP70-2 antibody.
• Purification: Protein A/G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC staining of FFPE human breast carcinoma tissue with HSP70-2 antibody (clone HSPA1B/7625). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human T-47D & HEK293 cell lysates using HSP70-2 antibody (clone HSPA1B/7625). Expected molecular weight ~70 kDa.

SDS-PAGE analysis of purified, BSA-free HSP70-2 antibody (clone HSPA1B/7625) as confirmation of integrity and purity.

Quick Database Links

UniProt

P0DMV9