HSP27 Mouse Monoclonal Antibody

SKU: orb1735104

Description

HSP27 Mouse Monoclonal Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: Western Blot 1:1000-1:2000, Immunofluorescence 1:200 – 1:500
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: B1E4
• Target: HSP27
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate-buffered solutions, pH 7.2, 50% Glycerol, 0.025% ProClin 300 was added as a preservative
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Anti-HSP27 antibody [9C2] at 1/1000 dilution, Lysates at 30 µg per lane. This blot was produced using a 12% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1735104 overnight at 4°C.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1735104 anti-HSP27 antibody [9C2] (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Quick Database Links

Gene Symbol

HSP27