HPD Antibody

SKU: orb1262616

Description

HPD Antibody

Research Area

Cell Biology, Metabolism Research, Signal Transduction

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Reactivity: Human, Mouse
• Application Notes:
  For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Immunogen: This HPD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 92-121 amino acids from the N-terminal region of human HPD.
• Target: HPD
• Molecular Weight: 45 kDa
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

4-hydroxyphenylpyruvate dioxygenase, 4-hydroxyphenylpyruvic acid oxidase, 4HPPD, HPD, HPPDase, HPD, PPD

Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.

Western blot analysis of HPD Antibody in mouse liver tissue lysates (35 ug/lane)

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with HPD Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Flow cytometric analysis of HepG2 cells using HPD Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Quick Database Links

Gene Symbol

HPD

UniProt

P32754

RefSeq:P32754