PD-1 Rabbit Polyclonal Antibody

• Catalog Number: orb13641
• Category: Antibodies
• Description: PD-1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC
• Predicted Reactivity: Bovine, Equine, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human PD-1 (201-288/288aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: Flow-Cyt=1μg /test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 55-60 kDa
• Target: PDCD1
• UniProt ID: Q15116
• RRID: AB_10749284
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Programmed cell death protein 1; CD279; CD279 anti
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

G Sinos, D Schizas, A Kapelouzou, M Frountzas, M Katsimpoulas, KS Mylonas The Novel Role of the Expression of Toll-like Receptors TLR-5, TLR-6, and TLR-9 and Associated Up-Regulation of Programmed Cell Death 1 Receptor (PD-1) and Its Ligand (PD-L1) in Lung Sepsis International Journal of Molecular Sciences, (2025)

PubMed:

Blank control (blue line): Mouse spleen cells (blue). Primary Antibody (green line): Rabbit Anti-PD-1/PE-CY7 Conjugated antibody, Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG-PE-CY7. Protocol, The cells were fixed with 70% ice-cold methanol overnight at 4°C. The cells were then incubated in 1X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PD-1) Polyclonal Antibody, Unconjugated (orb13641) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat spleen), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PD-1) Polyclonal Antibody, Unconjugated (orb13641) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Hela (Human) Cell Lysate at 30 ug, MOLT-4 (Human) Cell Lysate at 30 ug, Jurkat (Human) Cell Lysate at 30 ug, Primary: Anti-PD-1 (orb13641) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 32 kD, Observed band size: 55 kD.

Sample: Raji (Human) Cell Lysate at 30 ug, Thymus (Mouse) Lysate at 40 ug, Lymph node (Mouse) Lysate at 40 ug, Spleen (Mouse) Lysate at 40 ug, Raw264.7 (Mouse) Cell Lysate at 40 ug, Thymus (Rat) Lysate at 40 ug, Primary: Anti-PD-1 (orb13641) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55 kD, Observed band size: 58 kD.