HOXc8 Rabbit Polyclonal Antibody

SKU: orb10832

Description

HOXc8 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Rat
• Predicted Reactivity: Bovine, Equine, Mouse, Porcine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human HOXc8 (51-130/242aa)
• Target: HOXC8
• Molecular Weight: 27 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Hox-3.1; Homeo box 3A; Homeo box 8; Homeobox 3A; Homeobox C8; Homeobox protein Hox C8; Homeobox protein Hox-C8; Homeobox protein HoxC8; Homeobox protein R4; Homolog of mouse Hox 3.1; HOX 3; Hox 3.1 mouse homolog of; HOX 3A; HOX C8; Hox-3.1, MOUSE, HOMOLOG OF; Hox-3A; HOX3; HOX3A; HOXC 8; Hoxc-8; M31; HXC8_HUMAN.

Paraformaldehyde-fixed, paraffin embedded (Human cervical cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Histone H3) Polyclonal Antibody, Unconjugated (orb10832) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human cervical cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Hox-3.1) Polyclonal Antibody, Unconjugated (orb10832) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat small intestine, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer normal goat serum at 37°C for 30 min, Antibody incubation with (Hox-3.1) Polyclonal Antibody, Unconjugated (orb10832) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Brain (Rat) lysate at 30 ug, Liver (Rat) lysate at 30 ug, Primary: Anti-HOXc8 (orb10832) at 1:200, Secondary: HRP conjugated Goat-Anti-Rabbit IgG at 1:3000, Predicted band size: 27kD, Observed band size: 27kD, 43kD.

Sample: Liver (Rat) Lysate at 40 ug, Primary: Anti-HOXc8 (orb10832) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 27 kD, Observed band size: 27 kD.

Quick Database Links

Gene Symbol

HOXC8

UniProt

P31273