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Horse IgG F(ab')2 Antibody Fluorescein Conjugated

Horse IgG F(ab')2 Antibody Fluorescein Conjugated

Catalog Number: orb347160

DispatchUsually dispatched within 5-10 working days
$ 350.00
Catalog Numberorb347160
CategoryAntibodies
DescriptionHorse IgG F(ab')2 antibody (FITC)
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsFC, FLISA, IF
ReactivityEquine
IsotypeIgG
ImmunogenHorse IgG F(ab')2 fragment
Antibody TypeSecondary Antibody
Concentration1.5 mg/mL
Dilution rangeFLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
Form/AppearanceLyophilized
PurityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Horse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Horse IgG, Horse IgG F(ab’)2 and Horse Serum. No reaction was observed against Horse IgG F(c).
ConjugationFITC
StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Buffer/Preservatives0.01% (w/v) Sodium Azide
Alternative namesRabbit Anti-Horse IgG Fab2 Fragment fluorescein Co
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NoteFor research use only
Application notesThis product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Expiration Date12 months from date of receipt.
  • Horse IgG F(ab')2 Fluorescein Antibody [orb346184]

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    This product was prepared from normal serum by delipidation, salt fractionation and ion change chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Horse IgG, anti-Horse IgG F(ab’)2 and anti-Horse Serum. No reaction was observed against anti-Horse IgG F(c) or anti-Pepsin.

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