HLA-E Antibody / MHC class I antigen E

SKU: orb3066949

Description

Major histocompatibility complex (MHC) molecules, which include human leukocyte antigens (HLAs), form an integral part of the immune response system. They are cell-surface receptors that bind foreign peptides and present them to cytotoxic T lymphocytes (CTLs). MHC class I molecules consist of two polypeptide chains, an a or heavy chain and a non-covalently associated protein, b-2-Microglobulin. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes. HLA-A is a MHC class I heavy chain molecule that plays a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. HLA-B and HLA-C are proteins encoded by closely related genes that also exist in the MHC class I. HLA-E belongs to the HLA class I heavy chain paralogs. HLA-E is a heterodimer consisting of a heavy chain and a light chain. The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules.

Research Area

Immunology & Inflammation; Cell Biology

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: Immunohistochemistry (FFPE): 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the HLA-E antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2, kappa
• Clone No.: HLAE/9468
• Immunogen: A portion of amino acids 1-150 from human HLAE protein was used as the immunogen for the HLA-E antibody.
• Purification: Protein G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

UniProt

P13747