HLA-DR Antibody (MHC II)

SKU: orb2638563

Description

mAb IPO-10 defines the antigen, which appears on B cell progenitors following HLA-DR and preceding CD10, CD19, CD22, CD37 and cym. It is expressed on resting B cells and than reappears and persists in cytoplasm and on cell surface until cytoplasmic Ig appears. It is a useful antibody for diagnostics of neoplasms of B cell origins. It reacts with human B cell lines Daudi, Raji, Namalva, EB-3, RPMI-8226 (50% of cells). The mAb does not label T cell lines, blood granulocytes, thymocytes or bone marrow stromal fibroblasts. No significant changes are detected after PHA or ConA stimulation while LPS and PWM stimulated cultures after 18-48h show decreased number of antigen-positive cells but in final terms of cultivation antigen is expressed again. This mAb labels B cell leukemias and some lymphomas. Hairy cell leukemia strongly reacts and 70% of B cell CLL and some B-NHL were also positive. IPO-10 reacts with AMML cells and in a majority of Hodgkin's disease cases a significant percentage of affected lymph node cells were detected.

Research Area

Immunology & Inflammation; Cell Biology

Images & Validation

• Tested Applications: FACS, IF
• Dilution Range: Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the HLA-DR antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG3, kappa
• Clone No.: IPO-10
• Immunogen: Spleen cells from a patient with hairy cell leukemia were used as the immunogen for the HLA-DR antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

SDS-PAGE Analysis of Purified, BSA-Free HLA-DR Antibody (clone IPO-10). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

UniProt

P01903