HLA-DQA2 Antibody

• Catalog Number: orb1538964
• Category: Antibodies
• Description: HLA-DQA2 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, IHC-P, WB
• Reactivity: Bovine, Goat, Human, Sheep
• Immunogen: Peptide with sequence C-QSHGPSGQYTH, from the internal region of the protein sequence according to NP_064440.1.
• Concentration: 0.5 mg/ml
• Dilution range: IHC, IHC-P (3.75 µg/ml), Peptide-ELISA (1:16000), WB (0.1 - 0.3 µg/ml)
• Conjugation: Unconjugated
• Target: HLA-DQA2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Alternative names: HLA-DQA2, DX-ALPHA, DX alpha chain, MHC class II D
• Note: For research use only
• Application notes: Further information: Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Preliminary experiments gave bands at approx 35kD and 24kD in Human Lymph Node and Tonsil lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 28.0kD according to NP_064440.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
• Expiration Date: 12 months from date of receipt.

Goat Anti-HLA-DQA2 Antibody (0.3 µg/ml) staining of Human Bone Marrow lysate (35 µg protein in RIPA buffer). Detected by chemiluminescencence.