Histone H4 R3me1 antibody

• Catalog Number: orb420424
• Category: Antibodies
• Description: Histone H4 R3me1 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ChIP, IF, IHC, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Histone H4 [Monomethyl Arg3] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic monomethylated peptide surrounding Arginine 3 of human Histone H4.
• Concentration: 0.64 mg/ml
• Dilution range: ChIP: 2-5µg/million cells, IHC: 1:50, IF: 1:50, WB: 1:1000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Histone H4 [Monomethyl Arg3] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H4. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and rat. Predicted to react with most mammal species. Cross-reactivity with Histone H4 from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: P62805
• NCBI: NP_001029249
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Histone H4 monomethyl Arg3 antibody, H
• Note: For research use only
• Application notes: Anti-Histone H4 [Monomethyl Arg3] antibody is tested for Western Blot and Immunofluorescence. This antibody is useful in Dot Blot, Chromatin Immunoprecipitation, and Immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~13 kDa corresponding to Histone H4 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus antibody production in collaboration with Novus Biologicals.
• Expiration Date: 12 months from date of receipt.