HIST4H4 Antibody

SKU: orb420424

Description

Histone H4 R3me1 antibody

Images & Validation

• Tested Applications: ChIP, IF, IHC, WB
• Dilution Range: ChIP: 2-5µg/million cells, IHC: 1:50, IF: 1:50, WB: 1:1000
• Reactivity: Human, Mouse
• Application Notes:
  Anti-Histone H4 [Monomethyl Arg3] antibody is tested for Western Blot and Immunofluorescence. This antibody is useful in Dot Blot, Chromatin Immunoprecipitation, and Immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~13 kDa corresponding to Histone H4 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus antibody production in collaboration with Novus Biologicals.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Histone H4 [Monomethyl Arg3] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic monomethylated peptide surrounding Arginine 3 of human Histone H4.
• Target: HIST4H4
• Purity: Anti-Histone H4 [Monomethyl Arg3] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H4. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and rat. Predicted to react with most mammal species. Cross-reactivity with Histone H4 from other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 30% Glycerol; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 0.64 mg/ml
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Histone H4 monomethyl Arg3 antibody, H4R3me1, HIST2H4B, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4, HIST2H4A, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, histone cluster 4, H4, histone 4, H4

Immunofluorescence of Rabbit Anti-Histone H4 [Monomethyl Arg3] Antibody. Tissue: Nonmitotic, prophase, and telophase HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H4 [Monomethyl Arg3] antibody at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10000 for 45 min at RT. Localization: Histone H4 [Monomethyl Arg3] is nuclear and chromosomal. Staining: Histone H4 [Monomethyl Arg3] is expressed in green, nuclei are counterstained with DAPI (blue).

Western Blot of Rabbit Anti-Histone H4 [Monomethyl Arg3] Antibody. Lane 1: NIH-3T3 histone preps. Load: 30 µg per lane. Primary antibody: Histone H4 [Monomethyl Arg3] at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~13 kDa. Other band(s): None.

Quick Database Links

Gene Symbol

HIST4H4

UniProt

P62805

RefSeq (Protein):NP_001029249