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Histone H3 (tri methyl K9, phospho S10) Mouse Monoclonal Antibody
Catalog Number: orb1172876
| Catalog Number | orb1172876 |
|---|---|
| Category | Antibodies |
| Description | Histone H3 (tri methyl K9, phospho S10) Mouse Monoclonal Antibody |
| Target | H3C1 |
| Clonality | Monoclonal |
| Species/Host | Mouse |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
| Form/Appearance | Liquid |
| Concentration | 1mg/ml |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Tested applications | WB |
| Dilution range | WB=1:500-1:2000 |
| Antibody Type | Primary Antibody |
| Clone Number | G5E7 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Note | For research use only |
Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Mouse IgG H&L (HRP), Lysate: (-) HeLa, (+) HeLa + Nocodazole (100 ng/ml, 18hr) +Calyculin A (100nM, 1hr), Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 17 kDa, Observed MW: 17 kDa.
