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Catalog Number | orb1172853 |
---|---|
Category | Antibodies |
Description | Histone H3 (tri methyl K9, phospho S10) Rabbit Polyclonal Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC-Fr, IHC-P, WB |
Predicted Reactivity | Rat |
Reactivity | Human, Mouse |
Isotype | IgG |
Antibody Type | Primary Antibody |
Concentration | 1mg/ml |
Dilution range | WB=1:500-1:2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=0 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | H3C1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: (-) C2C12, (+) C2C12 + Nocodazole (100 ng/ml, 18hr) +Calyculin A (100nM, 1hr), Protein loading quantity: 20 µg, Exposure time: 30 s, Predicted MW: 17 kDa, Observed MW: 17 kDa.
Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: (-) JK, (+) JK + Etopside (100 ug/ml, 24hr) + Calyculin A (100nM, 1hr), Protein loading quantity: 20 µg, Exposure time: 30 s, Predicted MW: 17 kDa, Observed MW: 17 kDa.
Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: Mouse spleen, Mouse liver, Protein loading quantity: 20 µg, Exposure time: 30 s, Predicted MW: 17 kDa, Observed MW: 17 kDa.
Tissue: Human neuroblastoma, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:200, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1172853.
Tissue: Human neuroblastoma, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:200, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1172853.