Histone H2B (Crotonyl-Lys15 / Lys16 / Lys20) Recombinant Rabbit Monoclonal Antibody

SKU: orb1499337

Description

Histone H2B (Crotonyl-Lys15 / Lys16 / Lys20) Recombinant Rabbit Monoclonal Antibody

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: WB
• Dilution Range: WB=1:500-2000
• Reactivity: Human
• Predicted Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: 1D12
• Immunogen: KLH conjugated Synthesised acetylpeptide derived from human Histone H2B around the Crotonyl site of Lys15 / Lys16 / Lys20
• Target: H2BC3
• Molecular Weight: 14 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

H2BC3 | Histone H2B (cr-K15/K16/K20); cr-Histone H2B; crotonyl-Histone H2B; H2BK15CrK16CrK20Cr; cr-H2B; H2B.1; H2B/f; H2BFF; HIST1H2BB; H2B1B_HUMAN; H2BC3; H2B-clustered histone 3; Histone H2B.1; Histone H2B.f (H2B/f);

Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:2000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: (-): HeLa, (+): HeLa + sodium, butyrate (30mM, 4hr), Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 14kDa, Observed MW: 14kDa.

Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:2000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: (-): MCF-7, (+): MCF-7 + sodium, butyrate (50mM, 24hr) + trichostain A (500 ng/ml, 4 hr), Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 14kDa, Observed MW: 14kDa.

Quick Database Links

Gene Symbol

H2BC3

UniProt

P33778