Histone H2B (Crotonyl-Lys15 / Lys16 / Lys20) Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb1499337
• Category: Antibodies
• Description: Histone H2B (Crotonyl-Lys15 / Lys16 / Lys20) Recombinant Rabbit Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: 1D12
• Tested applications: WB
• Predicted Reactivity: Human
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised acetylpeptide derived from human Histone H2B around the Crotonyl site of Lys15 / Lys16 / Lys20
• Antibody Type: Recombinant Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 14 kDa
• Target: H2BC3
• UniProt ID: P33778
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Crotonyl-Histone H2B(Lys15/Lys16/Lys20); Crotonyl-
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:2000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: (-): HeLa, (+): HeLa + sodium, butyrate (30mM, 4hr), Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 14kDa, Observed MW: 14kDa.

Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:2000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: (-): MCF-7, (+): MCF-7 + sodium, butyrate (50mM, 24hr) + trichostain A (500 ng/ml, 4 hr), Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 14kDa, Observed MW: 14kDa.