Histone H2B (Acetyl K11) Mouse Monoclonal Antibody

SKU: orb1152019

Description

Histone H2B (Acetyl K11) Mouse Monoclonal Antibody

Images & Validation

• Tested Applications: WB
• Dilution range: WB=1:500-2000
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: H2C1
• Immunogen: KLH conjugated synthetic peptide derived from human Histone H2B (Acetyl K11)
• Target: H2BC3
• Molecular Weight: 14 kDa
• Purification: Affinity purified by Protein G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

H2BK11ac; Histone H2B(Acetyl Lys11); Acetyl Histone H2B(K12); Histone H2B/HIST1H2BD; H2B.1; H2B.1 B; H2B.b; H2B.c; H2B.d; H2B.e; H2B.f; H2B.j; H2B.q; H2BFB; H2BFC; H2BFD; H2BFE; H2BFF; H2BFJ; H2BFO; H2BFQ; H2BFS; HIRIP2; HIST1H2BB; HIST1H2BD; HIST1H2BH; HIST1H2BL; HIST1H2BM; HIST1H2BN; HIST2H2BE; Histone H2B; Histone H2B type 1 B; Histone H2B type 1 D; Histone H2B type 1 H; Histone H2B type 1 L; Histone H2B type 1 M; Histone H2B type 1 N; Histone H2B type 2 E; Histone protein; H2B GL105; H2B histone family member O; H2B histone family member S; histone H2B type 1-B; H2B1B_HUMAN.

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Mouse IgG H&L (HRP), Lysate: (-) HeLa, (+) HeLa + Sodium butyrate (30mM, 4hr), Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 14 kDa, Observed MW: 14 kDa.

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:500, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Mouse IgG H&L (HRP), Lysate: Mouse liver, Mouse spleen, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 14 kDa, Observed MW: 14 kDa.

IP of HeLa + Sodium butyrate (30mM, 4hr) cells extracts, IP ab incubation condition: orb1152019, 4°C overnight, 1:25, 1:100 dilution, WB primary ab incubation condition: orb1152019, room temperature 2h, 1:2000 dilution, Secondary ab: Anti-Mouse IgG for IP (HRP), Blocking buffer and Concentration: 5% NFDM/TBST, Diluting buffer and Concentration: 5% NFDM/TBST, Lane 1: 5% Input, Lane 2: IP with orb1152019 (1:25), Lane 3: IP with orb1152019 (1:100), Observed MW: 14 kDa, Exposure time: 60 s.

Quick Database Links

Gene Symbol

H2BC3

UniProt

P33778