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HIST1H2BB Antibody

SKU: orb416475

Description

This HIST1H2BB (Ab-16) Antibody is an unconjugated polyclonal product. It tagets HIST1H2BB using a Peptide sequence around site of Lys (16) derived from Human Histone H2B type 1-B as the immunogen. This antibody is suitable for ChIP, ELISA, IF, WB. Purification: Antigen Affinity Purified.

Research Area

Epigenetics & Chromatin

Images & Validation

Tested ApplicationsChIP, ELISA, IF, WB
Dilution rangeWB:1:500-5000, IF:1:50-200
ReactivityHuman, Mouse

Key Properties

HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenPeptide sequence around site of Lys (16) derived from Human Histone H2B type 1-B
TargetHIST1H2BB
PurificationAntigen Affinity Purified
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesPreservative: 0.03% Proclin 300. Constituents: 50% Glycerol
DisclaimerFor research use only

Alternative Names

Anti-Histone H2B type 1-B antibody, anti- Histone H2B.1 antibody, anti- Histone H2B.f antibody, anti- H2B/f antibody, anti- HIST1H2BB antibody, anti- H2BFF antibody

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HIST1H2BB Antibody

Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti- HIST1H2BB (Ab-16) antibody or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time

HIST1H2BB Antibody

Western blot aanalysis of Hela cell acid extracts, Jurkat cell acid extracts, Mouse brain tissue, Mouse liver tissue, Mouse kidney tissueusing HIST1H2BB (Ab-16) antibody

HIST1H2BB Antibody

Immunofluorescent analysis of Hela cells using HIST1H2BB (Ab-16) antibody

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
ChIP
Chromatin Immunoprecipitation
View Protocol

HIST1H2BB Antibody (orb416475)

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Available Sizes

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50 μl
$ 200.00
100 μl
$ 340.00
DispatchUsually dispatched within 5-10 working days
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