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Description
Research Area
Cell Biology, Molecular Biology
Images & Validation
−Item 1 of 3
| Tested Applications | WB |
|---|---|
| Reactivity | Human |
| Predicted Reactivity | Bovine, Canine, Guinea pig, Mouse, Rabbit, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Immunogen | The immunogen is a synthetic peptide directed towards the N terminal region of human HIST1H1E |
| Target | HIST1H1E |
| Protein Sequence | Synthetic peptide located within the following region: MSETAPAAPAAPAPAEKTPVKKKARKSAGAAKRKASGPPVSELITKAVAA |
| Molecular Weight | 22 kDa |
| Purification | Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Concentration | 0.5 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−H1E, H1.4, H1F4, RMNS, H1s-4, HIST1H1E, dJ221C16.5
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25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/ml of the antibody was used in this experiment.
Positive control (+): THP-1 (N30), Negative control (-): A549 (N03), Antibody concentration: 3 ug/ml.
WB Suggested Anti-HIST1H1E Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:62500, Positive Control: ACHN cell lysate.
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HIST1H1E Rabbit Polyclonal Antibody (orb583946)
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