HepPar1 Antibody [HepPar1]

SKU: orb1252388

Description

HepPar1 Antibody [HepPar1]

Images & Validation

• Tested Applications: IF, IHC-P
• Reactivity: Canine, Human
• Application Notes:
  Immunofluorescence: 0.5-1 µg/mLImmunohistochemistry (FFPE): 0.25-0.5 µg/mL for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Optimal dilution of the HepPar1 antibody should be determined by the researcher.1.Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: HepPar1
• Immunogen: Extract of a formalin-fixed, rejected-allograft of a human liver was used as the immunogen for the HepPar1 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Formalin-fixed, paraffin-embedded human hepatocellular carcinoma stained with HepPar1 antibody.

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