Hemoglobin beta F antibody

• Catalog Number: orb420239
• Category: Antibodies
• Description: Hemoglobin beta F antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 4B3.B5.F3.B7
• Tested applications: ELISA, WB
• Reactivity: Human
• Immunogen: Anti-Hemoglobin beta F Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near the N-terminus of Hb β-subunit conjugated to KLH.
• Concentration: 1.0mg/ml
• Dilution range: ELISA: 1:20,000, WB: 1ug/mL
• Form/Appearance: Liquid (sterile filtered)
• Purity: This protein A purified mouse monoclonal antibody reacts specifically with human HbF gamma isoform. Anti-HbF is purified from tissue culture supernatant by protein A purification. Blast analysis shows 100% homology to Human, Pan troglodytes, Pan paniscus, Gorilla gorilla gorilla, Hylobates lar, Macaca nemestrina, Macaca mulatta, Pongo pygmaeus, Pongo pygmaeus, Macaca fuscata fuscata, and Papio cynocephalus. This antibody does not react with the HbA, HbS, HbC, or HbA-2 isoforms.
• Conjugation: Unconjugated
• UniProt ID: P69891
• NCBI: NP_000550.2
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: mouse anti-HbF antibody, mouse anti-hemoglobin ant
• Note: For research use only
• Application notes: Anti-Hemoglobin beta F (MOUSE) antibody has been tested ELISA, SDS-Page, and Western Blotting. This antibody is designed for use in lateral flow. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 16 kDa.
• Expiration Date: 12 months from date of receipt.