Hemoglobin beta F Antibody

SKU: orb420238

Description

Hemoglobin beta F antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: ELISA: 1:20,000, WB: 1ug/mL
• Reactivity: Human
• Application Notes:
  Anti-Hemoglobin beta F (MOUSE) antibody has been tested ELISA, SDS-Page, and Western Blotting. This antibody is designed for use in lateral flow. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 16 kDa.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Clone No.: 4B3.B5.F3.B7
• Immunogen: Anti-Hemoglobin beta F Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near the N-terminus of Hb β-subunit conjugated to KLH.
• Purity: This protein A purified mouse monoclonal antibody reacts specifically with human HbF gamma isoform. Anti-HbF is purified from tissue culture supernatant by protein A purification. Blast analysis shows 100% homology to Human, Pan troglodytes, Pan paniscus, Gorilla gorilla gorilla, Hylobates lar, Macaca nemestrina, Macaca mulatta, Pongo pygmaeus, Pongo pygmaeus, Macaca fuscata fuscata, and Papio cynocephalus. This antibody does not react with the HbA, HbS, HbC, or HbA-2 isoforms.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/ml
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

mouse anti-HbF antibody, mouse anti-hemoglobin antibody, Gamma-1-globin, Hb F Agamma, Hemoglobin gamma-1 chain, Hemoglobin gamma-A chain, HBG1, HBG2, HbF Antibody, Sickle Cell Disease (SCD)

Western Blot of Mouse Anti-Hemoglobin beta F Antibody. Lane 1: Molecular Weight Ladder. Lane 2: HbA peptide conjugated to BSA. Lane 3: HbA-2 peptide conjugated to BSA. Lane 4: HbC peptide conjugated to BSA. Lane 5: HbF peptide conjugated to BSA. Lane 6: HbS peptide conjugated to BSA. Load: 50 ng per lane. Primary antibody: Anti-HbF antibody at 1 µg/ml overnight at 4°C. Secondary antibody: Rabbit Anti-Mouse secondary antibody at 1:40000 for 30 min at RT. Block: orb348644 for 30 min RT. Predicted/Observed: Reactivity seen in Lane 5 specific to HbF only.

Quick Database Links

UniProt

P69891

RefSeq (Protein):NP_000550.2