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Hamster IgG F(ab')2

SKU: orb2652758

Description

Hamster IgG F(ab')2

Research Area

Protein Biochemistry

Images & Validation

Tested ApplicationsSDS-PAGE
Application Notes
HAMSTER IgG F(ab')2 fragment has been tested by SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments. Reconstitution Buffer: Restore with deionized water (or equivalent). Reconstitution Volume: 1.0 mL

Key Properties

SourceGolden Syrian Hamster
Biological OriginGolden Syrian Hamster
IsotypeIgG F(ab')2
ConjugationUnconjugated
PurityThis product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Golden Syrian Hamster IgG, anti-Golden Syrian Hamster IgG F(ab’)2 and anti-Golden Syrian Hamster Serum. No reaction was observed against anti-Golden Syrian Hamster IgG F(c) or anti-Pepsin.

Storage & Handling

StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/AppearanceLyophilized
Buffer/PreservativesPreservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration10.0 mg/mL
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Hamster IgG F(ab')2 Fragment, Hamster IgG Fab2 Fragment

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Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
View Guide
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Hamster IgG F(ab')2 (orb2652758)

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