F(ab')2 Golden Syrian Hamster IgG (H&L) Antibody

SKU: orb348145

Description

F(ab')2 Golden Syrian Hamster IgG (H&L) antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Reactivity: Golden Hamster
• Application Notes:
  F(ab')2 Anti-Golden Syrian Hamster IgG Antibody has been tested by SDS-PAGE and dot blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 µg of antibody. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG F(ab')2
• Immunogen: Golden Syrian Hamster IgG whole molecule
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Golden Syrian Hamster IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Golden Syrian Hamster IgG and Golden Syrian Hamster Serum. No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c). Limited reactivity will occur with Armenian Hamster IgG.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Rabbit F(ab')2 Anti-Golden Syrian & Armenian Hamster IgG Antibody, Rabbit F(ab')2 Anti-Hamster IgG Antibody, Rabbit Fab2 Anti-Hamster IgG Antibody

ELISA results of purified Rabbit F(ab')2 Anti-Golden Syrian Hamster IgG Antibody tested against purified Hamster IgG (Green Line). Each well was coated in duplicate with 1.0 µg of Hamster IgG (p/n orb2652759). The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer, Goat Anti-Rabbit HRP conjugated (p/n orb347654), and TMB substrate (p/n orb348651).