GZMB Antibody

• Catalog Number: orb1263785
• Category: Antibodies
• Description: GZMB Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This GZMB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 175-203 amino acids from the C-terminal region of human GZMB.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 28 kDa
• Target: GZMB
• UniProt ID: P10144
• NCBI: P10144
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Granzyme B, C11, CTLA-1, Cathepsin G-like 1, CTSGL
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of GZMB Antibody in HL-60 cell line lysates (35 ug/lane)

GZMB Antibody IHC analysis in formalin fixed and paraffin embedded human Lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of GZMB using rabbit polyclonal GZMB Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GZMB gene.