Guinea Pig Serum Antibody Peroxidase Conjugated

SKU: orb2652794

Description

Guinea Pig Serum Antibody Peroxidase Conjugated

Research Area

Neuroscience

Images & Validation

• Tested Applications: DOT
• Dilution Range: ELISA: User Optimized, IHC: User Optimized, WB: User Optimized
• Reactivity: Guinea pig
• Application Notes:
  Anti-Guinea Pig Serum Peroxidase Conjugated has been tested by dot blot and is suitable to be assayed against 1.0 ug of Guinea Pig IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Guinea Pig serum proteins
• Purity: Guinea Pig Serum is an IgG fraction antibody purified from polyspecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Rabbit Serum. Multiple precipitin arcs were observed against Guinea Pig Serum.
• Conjugation: HRP

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

Rabbit anti-Guinea Pig IgG Peroxidase Conjugated Antibody, Rabbit anti-Guinea Pig IgG HRP Conjugated Antibody, Rabbit anti-Guinea Pig IgG Peroxidase Conjugated Antibody, Rabbit anti-Guinea Pig IgG HRP Conjugated Antibody, Rabbit anti-Guinea Pig serum Peroxidase Conjugated Antibody, Rabbit anti-Guinea Pig serum HRP Conjugated Antibody

Dot Blot of Rabbit Anti-Guinea Pig Peroxidase Conjugated Antibody. Lane 1: 140 ng. Lane 2: 46 ng. Lane 3: 15.6 ng. Lane 4: 5.2 ng. Lane 5: 1.72 ng. Secondary Antibody: Rb-a-Guinea Pig HRP at 1 µg/ml. Blocking Buffer: BlockOut orb348644 for 30 min at RT.